Coding

Part:BBa_K3880009

Designed by: Honglei Jin   Group: iGEM21_GDSYZX   (2021-09-30)


Sep39(protease)

Sep39 is a major protease involved in SCUT-3’s keratin hydrolysis, and previous work showed that its overexpression could increase SCUT-Osep39’s keratinase activity. To further improve the degradation efficiency without influencing the growth rate, we attempt to balance the expression quantity of CDO1 and Sep39 in SCUT-3 through replacing the constitutive promoter with well-characterized promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 482
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 121
    Illegal NgoMIV site found at 661
    Illegal NgoMIV site found at 796
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None